Somatic embryogenesis from cell cultures of Medicago sativa LI The role of amino acid additions to the regeneration medium (Record no. 47277)

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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-13075
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Title Somatic embryogenesis from cell cultures of Medicago sativa LI The role of amino acid additions to the regeneration medium
490 0# - SERIES STATEMENT
Volume/sequential designation Plant Science Letters, 34, p.165-174, 1984
520 3# - SUMMARY, ETC.
Summary, etc. The effect of adding amino acids to cultures of alfalfa (Medicago sativa L.)undergoing in vitro somatic embryogenesis has been investigated. By supplementing Schenk-Hildebrandt (SH)medium with 50--300 mM L-proline, somatic embryogenesis was stimulated by 3-fold when compared with SH medium without proline. Several other proline analogs were also effective. Alanine, glutamine, arginine, lysine, serine, asparagine and ornithine also stimulated the numbers of somatic embryos formed in alfalfa cultures. The structural quality of embryos was also enhanced by glutamine, arginine or alanine in the regeneration medium. Both embryo size and conversion of embryos to plantlets were increased by these amino acids. It is suggested that adding proline with other amino acids which stimulate embryo conversion may be one method for achieving high frequency and high quality somatic embryogenesis.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SOMATIC EMBRYOGENESIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ALFALFA
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Topical term or geographic name entry element PROLINE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element AMINO ACIDS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Stuart, D.A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Strickland, S.G.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/18XuwaZhUsdOEJLlWdw70-RDqp7WXXlxr/view?usp=drivesdk">https://drive.google.com/file/d/18XuwaZhUsdOEJLlWdw70-RDqp7WXXlxr/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-13075 25.06.2025 25.06.2025 Documentos solicitados