Arabidopsis thaliana as a model system in somatic cell genetics II. Cell suspension culture (Record no. 47282)

MARC details
000 -LEADER
fixed length control field 01439nam a2200181Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625153915.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-13080
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250602s9999 xx |||||s2 |||| ||und|d
245 10 - TITLE STATEMENT
Title Arabidopsis thaliana as a model system in somatic cell genetics II. Cell suspension culture
490 0# - SERIES STATEMENT
Volume/sequential designation Plant Science Letters, 8, p.7-15, 1977
520 3# - SUMMARY, ETC.
Summary, etc. A cell culture system providing a suitable source of free cells for in vitro genetic manipulations is presented. The system is based on the correlation between dissociation rate and growth pattern in suspension cultures. This correlation is used to control the balance between cell division and cell dissociation. 2,4-Dichlorophenoxyacetic acid (2,4-D)at a concentration of 1 mg/1 is estimated to be the most efficient dissociating factor. The culture is permanently maintained in the late log phase by adding fresh medium to the biomass at a well-defined ratio; thus, cells are kept growing and cell dissociation is favoured. This makes it possible to use large cell aggregates s a continuous source of single cells and of small cell aggregates. A yield of 0.8 to 1.8.106 viable cells per ml is obtained.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Negrutiu, I.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Jacobs, M.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1EBQ6ldUeRMhOwInJ9iM8Rrq8VibxH5Kl/view?usp=drivesdk">https://drive.google.com/file/d/1EBQ6ldUeRMhOwInJ9iM8Rrq8VibxH5Kl/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
Holdings
Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-13080 25.06.2025 25.06.2025 Documentos solicitados