Assessment of the diagnostic potential of Immmunocapture-PCR and Immuno-PCR for Citrus Variegated Chlorosis (Record no. 47384)

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fixed length control field 01951nam a2200301Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625153917.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-13182
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245 10 - TITLE STATEMENT
Title Assessment of the diagnostic potential of Immmunocapture-PCR and Immuno-PCR for Citrus Variegated Chlorosis
490 0# - SERIES STATEMENT
Volume/sequential designation Journal of MicroBiological Methods, 75(2), p.302-307, 2008
520 3# - SUMMARY, ETC.
Summary, etc. Xylella fastidiosa causes significant losses in many economically important crops. An efficient pathogen detection system is critical for epidemiology studies, particularly when large sample size is involved. In this study we report the development of immunomolecular assays like Immmunocapture-PCR and Immuno-PCR for direct detection of X. fastidiosa without DNA isolation. Whereas the reactivity of ELISA and PCR ranged from 106 to 104 bacterial cells, the IC-PCR sensitivity was up to 103 and the detection limit of I-PCR was up to 101 bacterial cells. These methods can use either plant sample extracts or cultivated media, and show no cross reaction for any other endophytic citrus-bacteria. Therefore, IC-PCR and I-PCR assays provide an alternative for quick and very sensitive methods to screening X. fastidiosa, with the advantage of not requiring any concentration or DNA purification steps while still allowing an accurate diagnosis of CVC.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element XYLELLA FASTIDIOSA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CITRUS VARIEGATED CHLOROSIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element IMMUNOCAPTURE-PCR
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element IMMUNO-PCR
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ELISA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PCR
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Peroni, L.A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ribeiro Dos Reis, J.R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Coletta-Filho, H.D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Alves De Souza, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Machado, M.A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Stach-Machado, D.R.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1tA63hQoQMhooVIB3mkxuJOGe8-irs8vz/view?usp=drivesdk">https://drive.google.com/file/d/1tA63hQoQMhooVIB3mkxuJOGe8-irs8vz/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-13182 25.06.2025 25.06.2025 Documentos solicitados