Isolation and characterization of a eDNA that encodes ECP31, an embryogenic-cell protein from carrot (Record no. 47426)
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| 000 -LEADER | |
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| fixed length control field | 02470nam a2200313Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625153918.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-13225 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Isolation and characterization of a eDNA that encodes ECP31, an embryogenic-cell protein from carrot |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Plant Molecular Biology, 19, p.239-249, 1992 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | A full-length eDNA for ECP31, an embryogenic cell protein from carrot (Daucus carota L.)with a Mr of 31000 (Kiyosue T, Satoh S, Kamada H, Harada H (1991)Plant Physiol 95: 1077-1083), was isolated from a eDNA library prepared from embryogenic cells using PCR-amplified DNA as a probe. The genomic Southern blot analysis revealed that there are two or three genes for ECP31 in the carrot gehome. The transcripts of ECP31 accumulated in the peripheral regions of clusters of embryogenic cells and disappeared in the course of somatic embryogenesis that was induced by transfer of the embryogenie cells to auxin-free media. The cDNA encodes a polypeptide of 256 amino acids, and the calculated molecular weight of this polypeptide is 26 111. The deduced amino acid sequence shows a high degree (62.2~o)of similarity to that of a protein that is abundant during late embryogenesis of cotton (LEA D34; Baker JC, Steele C, Dure III (1988)Plant Mol Biol 11: 227-291). The RNAs for ECP31 started to accumulate in zygotic embryos at a late stage ofembryogenesis but were undetectable in mature embryos within 24 h after imbibition of seeds. In dry fruits (seeds), the transcripts were detected only in zygotic embryos by in situ hybridization. The level of ECP31 transcripts increased after treatment with abscisic acid (ABA)in torpedo-shaped somatic embryos but not in seven-day-old seedlings. These results suggest that both embryo-specific factor(s)and ABA are involved in the expression of the gene for ECP31. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ABA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | DAUCUS CAROTA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ECP31 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | GENE EXPRESSION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | LEA CLONE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | SOMATIC EMBRYOGENESIS |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kiyosue, T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yamaguchi-Shinozaki, K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Shinozaki, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Higashi, K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Satoh, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kamada, H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Harada, H. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1ml4lXoCcHmj5ZutTpuFrA3frkxQnm9r-/view?usp=drivesdk">https://drive.google.com/file/d/1ml4lXoCcHmj5ZutTpuFrA3frkxQnm9r-/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-13225 | 25.06.2025 | 25.06.2025 | Documentos solicitados |