Development of high-performance liquid chromatographic systems for the separation of radiolabelled carotenes and precursors formed in specific enzymatic reactions (Record no. 47438)

MARC details
000 -LEADER
fixed length control field 01692nam a2200193Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625153918.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-13237
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250602s9999 xx |||||s2 |||| ||und|d
245 10 - TITLE STATEMENT
Title Development of high-performance liquid chromatographic systems for the separation of radiolabelled carotenes and precursors formed in specific enzymatic reactions
490 0# - SERIES STATEMENT
Volume/sequential designation Journal of Chromatography, 645, p.265-272, 1993
520 3# - SUMMARY, ETC.
Summary, etc. High-performance liquid chromatographic (HPLC)systems were developed to separate radiolabelled carotenes and precursors formed in the following carotenogenic enzyme reactions: geranylgeranyl pyrophosphate synthase, phytoene synthase, phytoene desaturase(s), lycopene cyclase and p-carotene hydroxylase. Separations were carried out on reversed- (C,,)and normal-phase columns, using mobile phase mixtures of acetonitrile, methanol and hexane with appropriate modifiers. An isocratic mode of elution was employed throughout, although in several instances isocratic steps were necessary to achieve the desired resolution. The methods developed are specific for each enzyme reaction, resolving substrate from its reaction products and any interfering radiolabelled compounds, thus permitting reliable and accurate determination of enzyme activities. The new separation systems will facilitate further studies on the characterization of these proteins.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Fraser, P.D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Albrecht, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sandmann, G.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1qPEsyTevS6eN2VXBDDi6OixuS4W2NMDN/view?usp=drivesdk">https://drive.google.com/file/d/1qPEsyTevS6eN2VXBDDi6OixuS4W2NMDN/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
Holdings
Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-13237 25.06.2025 25.06.2025 Documentos solicitados