MARC details
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02862nam a2200349Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625153918.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-13238 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Two SERK genes are markers of pluripotency in Cyclamen persicum Mill. |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Journal of Experimental Botany, doi:10.1093/jxb/err295, 2011 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
The genetic basis of stem cell specification in somatic embryogenesis and organogenesis is still obscure. SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK)genes are involved in embryogenesis and organogenesis in numerous species. In vitro culture of Cyclamen persicum immature ovules provides a system for investigating stem cell formation and maintenance, because lines forming either organs or embryos or callus without organs/embryos are available for the same cultivar and plant growth regulator conditions. The present aim was to exploit this property of cyclamen cultures to understand the role of SERK(s)in stem cell formation and maintenance in somatic embryogenesis and organogenesis in vitro, in comparison with expression in planta. CpSERK1 and CpSERK2 were isolated from embryogenic callus. CpSERK1 and CpSERK2 levels by RT-PCR showed that expression is high in embryogenic, moderate in organogenic, and null in recalcitrant calli. in situ hybridizations showed that the expression of both genes started in clumps of pluripotent stem cells, from which both pre-embryogenic aggregates and organ meristemoids derived, and continued in their trans-amplifying, meristem-like, derivatives. Expression declined in organ meristemoids, in parallel with a partial loss of meristematization. In mature somatic embryos, and in shoot and root primordia, CpSERK1 and CpSERK2 were expressed in meristems, and similar patterns occurred in zygotic embryo and primary meristems in planta. The results point to SERK1 and SERK2 as markers of pluripotency in cyclamen. It is proposed that the high expression of these genes in the trans-amplifying derivatives of the stem cells maintains a pluripotent condition leading to totipotency and, consequently, somatic embryogenesis. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CYCLAMEN PERSICUM |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
MERISTEMS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PLURIPOTENCY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
STEM CELLS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
SOMATIC EMBYROGENESIS RECEPTOR-LIKE KINASE (SERK) |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
TOTIPOTENCY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
TRANS-AMPLIFYING CELLS. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Savona, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Mattioli, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Falasca, N.G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Della Rovere, F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Costantino, P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
De Vries, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Ruffoni, B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Ruffoni, B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Altamura, M.M. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1ptIlI4mCOuVT13YZW5G_BCsLjEkUc1-5/view?usp=drivesdk">https://drive.google.com/file/d/1ptIlI4mCOuVT13YZW5G_BCsLjEkUc1-5/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
