Two uncommon phospholipase D isoenzymes from poppy seedlings (Papaver somniferum L.) (Record no. 47451)

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control field 20250625153919.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-13250
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Title Two uncommon phospholipase D isoenzymes from poppy seedlings (Papaver somniferum L.)
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Volume/sequential designation Biochimica et Biophysica Acta, 1631(2), p.153-159, 2003
520 3# - SUMMARY, ETC.
Summary, etc. Phospholipase D (PLD)has been detected in seedlings of Papaver somniferum L. cv. Lazu´ r (Papaveraceae). Purification of the enzyme revealed the existence of two forms of PLD (named as PLD-A and PLD-B). The two enzymes strongly differ in their catalytic properties. The pH optima were found at pH 8.0 for PLD-A and at pH 5.5 for PLD-B. While both enzymes show hydrolytic activity toward phosphatidylcholine (PC)and phosphatidyl-p-nitrophenol (PpNP), PLD-B only was able to catalyze the exchange of choline in PC by glycerol. Both enzymes were activated by Ca2 + ions with an optimum concentration of 10 mM. In contrast to PLDs from other plants, LD-B was still more activated by Zn2 + ions with an optimum concentration of 5 mM. The apparent molecular masses of PLD-A and PLD-B, derived from sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE), were estimated to be 116.4 and 114.1 kDa. N-terminal protein sequencing indicated N-terminal blockage in both cases. The isoelectric points were found to be 8.7 for PLD-A and 6.7 for PLD-B. Both enzymes were shown to be N-linked glycoproteins. This paper is the first report on PLD in poppy and indicates some important differences of the two enzyme forms to other PLDs known so far.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PHOSPHOLIPASE D
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PAPAVER SOMNIFERUM
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Topical term or geographic name entry element PURIFICATION
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Topical term or geographic name entry element ZN-ACTIVATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TRANSPHOSPHATIDYLATION
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Oblozinsky, M.
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Personal name Schoeps, R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ulbrich-Hofmann, R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Bezakova, L.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1Xmvhn1XCXbNqiSWKDEylY0DIrqQh8SGu/view?usp=drivesdk">https://drive.google.com/file/d/1Xmvhn1XCXbNqiSWKDEylY0DIrqQh8SGu/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-13250 25.06.2025 25.06.2025 Documentos solicitados