Mining of the Catharanthus roseus Genome Leads to Identification of a Biosynthetic Gene Cluster for Fungicidal Sesquiterpenes (Record no. 47484)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 02201nam a2200241Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625153919.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-13283 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Mining of the Catharanthus roseus Genome Leads to Identification of a Biosynthetic Gene Cluster for Fungicidal Sesquiterpenes |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Journal of Natural Products, 84, p.2709-2716, 2021 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Characterization of cryptic biosynthetic gene clusters (BGCs)from microbial genomes has been proven to be a powerful approach to the discovery of new natural products. However, such a genome mining approach to the discovery of bioactive plant metabolites has been muted. The plant BGCs characterized to date encode pathways for antibiotics important in plant defense against microbial pathogens, providing a means to discover such phytoalexins by mining plant genomes. Here is reported the discovery and characterization of a minimal BGC from the medicinal plant Catharanthus roseus, consisting of an adjacent pair of genes encoding a terpene synthase (CrTPS18)and cytochrome P450 (CYP71D349). These two enzymes act sequentially, with CrTPS18 acting as a sesquiterpene synthase, producing 5-epi-jinkoheremol (1), which CYP71D349 further hydroxylates to debneyol (2). Infection studies with maize revealed that 1 and 2 exhibit more potent fungicidal activity than validamycin. Accordingly, this study demonstrates that characterization of such cryptic plant BGCs is a promising strategy for the discovery of potential agrochemical leads. Moreover, despite the observed absence of 1 and 2 in C. roseus, the observed transcriptional regulation is consistent with their differential fungicidal activity, suggesting that such conditional coexpression may be sufficient to drive BGC assembly in plants. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Liang, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | An, T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhu, J. X. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Chen, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhu, J. H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Peters, R. J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zi, J. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1jQAKkuJwlDDPBGqaexqFSAEmFKlvJPue/view?usp=drivesdk">https://drive.google.com/file/d/1jQAKkuJwlDDPBGqaexqFSAEmFKlvJPue/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-13283 | 25.06.2025 | 25.06.2025 | Documentos solicitados |