Use of a Secretion Trap Screen in Pepper Following Phytophthora capsici Infection Reveals Novel Functions of Secreted Plant Proteins in Modulating Cell Death (Record no. 47550)

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control field 20250625153921.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-13350
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Title Use of a Secretion Trap Screen in Pepper Following Phytophthora capsici Infection Reveals Novel Functions of Secreted Plant Proteins in Modulating Cell Death
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Volume/sequential designation Molecular Plant-Microbe Interaction, 24(6), p.671-684, 2011
520 3# - SUMMARY, ETC.
Summary, etc. In plants, the primary defense against pathogens is mostly inducible and associated with cell wall modification and defense-related gene expression, including many secreted proteins. To study the role of secreted proteins, a yeastbased signal-sequence trap screening was conducted with the RNA from Phytophthora capsici-inoculated root of Capsicum annuum 'Criollo de Morelos 334' (CM334). In total, 101 Capsicum annuum secretome (CaS)clones were isolated and identified, of which 92 were predicted to have a secretory signal sequence at their N-terminus. To identify differences in expressed CaS genes between resistant and susceptible cultivars of pepper, reverse Northern blots and real-time reverse-transcription polymerase chain reaction were performed with RNA samples isolated at different time points following P. capsici inoculation. In an attempt to assign biological functions to CaS genes, we performed in planta knock-down assays using the Tobacco rattle virusbased gene-silencing method. Silencing of eight CaS genes in pepper resulted in suppression of the cell death induced by the non-host bacterial pathogen (Pseudomonas syringae pv. tomato T1). Three CaS genes induced phenotypic abnormalities in silenced plants and one, CaS259 (PR4-l), caused both cell death suppression and perturbed phenotypes. These results provide evidence that the CaS genes may play important roles in pathogen defense as well as developmental processes.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yeom, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Baek, H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Oh, S.
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Personal name Kang, W.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Lee, S.J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Lee, J.M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Seo, E.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Seo, E.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kim, B.D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Choi, D.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1HWaRIc7wlLgRQZwJM2oOb_vTZ0QhxnFA/view?usp=drivesdk">https://drive.google.com/file/d/1HWaRIc7wlLgRQZwJM2oOb_vTZ0QhxnFA/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-13350 25.06.2025 25.06.2025 Documentos solicitados