Comparison of alamar blue and MTT assays for high through-put screening (Record no. 47581)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01539nam a2200277Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625153921.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-13381 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Comparison of alamar blue and MTT assays for high through-put screening |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Toxicology in Vitro, 18(5), p.703-710, 2004 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The performance of alamar blue and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT)cell viability assays in a high through-put format were compared. A total of 117 drugs chosen for their wide range of therapeutic areas were screened at 10 lM using both assays in human hepatoma cell line HepG2. Except for terfenadine and astemizole, which performed consistently in both assays, the alamar blue assay was slightly more sensitive than the MTT assay for most compounds. The MTT assay was less sensitive detecting an effect for daunorubicin and trifluoperazine. Seven drugs, astemizole, daunorubicin, ellipticine, fluphenazine, terfenadine, thioridazine and trifluoperazine, had percent viability results of 55 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | HEPG2 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ALAMAR BLUE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | MTT |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ASSAY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | HTS |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hamid, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rotshteyn, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rabadi, L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Parikh, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Bullock, P. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1TWxP4sWvBek167qpxJ2Wwg7WFbEhZHMj/view?usp=drivesdk">https://drive.google.com/file/d/1TWxP4sWvBek167qpxJ2Wwg7WFbEhZHMj/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-13381 | 25.06.2025 | 25.06.2025 | Documentos solicitados |