Reengineering a Tryptophan Halogenase To Preferentially Chlorinate a Direct Alkaloid Precursor (Record no. 47641)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 01880nam a2200193Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625153922.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-13441 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Reengineering a Tryptophan Halogenase To Preferentially Chlorinate a Direct Alkaloid Precursor |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Journal of the American Chemical Society, 133, p.19346-19349, 2011 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Installing halogens onto natural products can generate compounds with novel or improved properties. Notably, enzymatic halogenation is now possible as a result of the discovery of several classes of halogenases; however, applications are limited because of the narrow substrate specificity of these enzymes. Here we demonstrate that the flavin-dependent halogenase RebH can be engineered to install chlorine preferentially onto tryptamine rather than the native substrate tryptophan. Tryptamine is a direct precursor to many alkaloid natural products, including approximately 3000 monoterpene indole alkaloids. To validate the function of this engineered enzyme in vivo, we transformed the tryptamine-specific RebH mutant (Y455W)into the alkaloid-producing plant Madagascar periwinkle (Catharanthus roseus)and observed the de novo production of the halogenated alkaloid 12-chloro-19, 20-dihydroakuammicine. While wild-type (WT)RebH has been integrated into periwinkle metabolism reviously, the resulting tissue cultures accumulated substantial levels of 7-chlorotryptophan. Tryptophan decarboxylase, the enzyme that converts tryptophan to tryptamine, accepts 7-chlorotryptophan at only 3 |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Glenn, W.S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Nims, E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | O'Connor, S.E. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/19PxNJeIqbel5opp81bvUJV-CshlKtpCn/view?usp=drivesdk">https://drive.google.com/file/d/19PxNJeIqbel5opp81bvUJV-CshlKtpCn/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-13441 | 25.06.2025 | 25.06.2025 | Documentos solicitados |