Large Scale Identification of Genes Involved in Plant-Fungal Interactions Using Illumina's Sequencing-by-Synthesis Technology (Record no. 47644)
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| fixed length control field | 02396nam a2200313Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625153922.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-13444 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Large Scale Identification of Genes Involved in Plant-Fungal Interactions Using Illumina's Sequencing-by-Synthesis Technology |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Fungal Genomics: Methods and Protocols, Methods in Molecular Biology, 722, p.167-178, 2011 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Deep transcriptome profiling of pathogen-infected tissues enhances the understanding of molecular mechanisms underlying host-pathogen interactions. Illumina's next generation sequencing technology sequencing-by-synthesis (SBS)is a powerful tool to rapidly sequence genomes and transcriptomes at an affordable rate. We modified the procedure for SBS library construction to significantly increase the efficiency of library construction. Using our improved method, two Sclerotinia homoeocarpa libraries were constructed from mycelia grown in potato dextrose broth (PDB)or potato dextrose agar (PDA)for 96 h, respectively, and two creeping bentgrass libraries were constructed from leaves 96 h after inoculation with S. homoeocarpa or water sprayed, respectively. About 4-7 million mRNA signatures were sequenced from each library. Sequence analysis using BLAST was performed against sequenced fungal genomes and rice genomic sequence to identify the expressed genes in both S.omoeocarpa mycelia and creeping bentgrass. Bioinformatic analysis identified many expressed genes in the pathogen and host. A public database to access the sequence data was developed at http://www.dstidb.org. Our results demonstrate how SBS technology can unravel transcriptome complexity during the creeping bentgrass- S. homoeocarpa interaction. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | SEQUENCING-BY-SYNTHESIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | TRANSCRIPTOME ANALYSIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CREEPING BENTGRASS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | SCLEROTINIA HOMOEOCARPA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | BIOINFORMATICS |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Venu, R.C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhang, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Weaver, B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Carswell, P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mitchell, T.K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Meyers, B.C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Boehm, M.J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Boehm, M.J. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1S_favdxZxW_F6KbQh1xbNhsiOnHZX3qY/view?usp=drivesdk">https://drive.google.com/file/d/1S_favdxZxW_F6KbQh1xbNhsiOnHZX3qY/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-13444 | 25.06.2025 | 25.06.2025 | Documentos solicitados |
