Large Scale Identification of Genes Involved in Plant-Fungal Interactions Using Illumina's Sequencing-by-Synthesis Technology (Record no. 47644)

MARC details
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fixed length control field 02396nam a2200313Ia 4500
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control field MX-MdCICY
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control field 20250625153922.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-13444
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Title Large Scale Identification of Genes Involved in Plant-Fungal Interactions Using Illumina's Sequencing-by-Synthesis Technology
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Volume/sequential designation Fungal Genomics: Methods and Protocols, Methods in Molecular Biology, 722, p.167-178, 2011
520 3# - SUMMARY, ETC.
Summary, etc. Deep transcriptome profiling of pathogen-infected tissues enhances the understanding of molecular mechanisms underlying host-pathogen interactions. Illumina's next generation sequencing technology sequencing-by-synthesis (SBS)is a powerful tool to rapidly sequence genomes and transcriptomes at an affordable rate. We modified the procedure for SBS library construction to significantly increase the efficiency of library construction. Using our improved method, two Sclerotinia homoeocarpa libraries were constructed from mycelia grown in potato dextrose broth (PDB)or potato dextrose agar (PDA)for 96 h, respectively, and two creeping bentgrass libraries were constructed from leaves 96 h after inoculation with S. homoeocarpa or water sprayed, respectively. About 4-7 million mRNA signatures were sequenced from each library. Sequence analysis using BLAST was performed against sequenced fungal genomes and rice genomic sequence to identify the expressed genes in both S.omoeocarpa mycelia and creeping bentgrass. Bioinformatic analysis identified many expressed genes in the pathogen and host. A public database to access the sequence data was developed at http://www.dstidb.org. Our results demonstrate how SBS technology can unravel transcriptome complexity during the creeping bentgrass- S. homoeocarpa interaction.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SEQUENCING-BY-SYNTHESIS
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Topical term or geographic name entry element TRANSCRIPTOME ANALYSIS
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Topical term or geographic name entry element CREEPING BENTGRASS
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Topical term or geographic name entry element SCLEROTINIA HOMOEOCARPA
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Topical term or geographic name entry element BIOINFORMATICS
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Personal name Venu, R.C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zhang, Y.
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Personal name Weaver, B.
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Personal name Carswell, P.
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Personal name Mitchell, T.K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Meyers, B.C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Boehm, M.J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Boehm, M.J.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1S_favdxZxW_F6KbQh1xbNhsiOnHZX3qY/view?usp=drivesdk">https://drive.google.com/file/d/1S_favdxZxW_F6KbQh1xbNhsiOnHZX3qY/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-13444 25.06.2025 25.06.2025 Documentos solicitados