MARC details
| 000 -LEADER |
|---|
| fixed length control field |
02909nam a2200289Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20250625153923.0 |
| 040 ## - CATALOGING SOURCE |
|---|
| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
|---|
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-13491 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Mechanism of Retarded DNA Degradation and Prokaryotic Origin of DNases in Nonsterile Soils |
| 490 0# - SERIES STATEMENT |
|---|
| Volume/sequential designation |
Systematic and Applied MicroBiology, p.513-521, 1997 |
| 520 3# - SUMMARY, ETC. |
|---|
| Summary, etc. |
DNA released from organisms exits at considerable quantities in water, sediment and soil. Such DNA can serve as genetic information for transformable bacteria. It is not clear so far how DNA persists in an environment such as soil where DNase activity is present. The fate of linear duplex DNA (41.5 kbp)introduced into non-sterile soil microcosms was followed by monitoring the distribution of DNA to solid and liquid soil constituents and by measuring the degradation of DNA in each of the two fractions by DNases produced by microorganisms indigenous to the soils. Three non-sterile soils collected from agricultural field plots including a brown earth, a loamy soil and a podzol were examined using [3H]-thymidine- labeled DNA. Depending on the soil type, DNA adsorption reached an equilibrium within 1 to 3 h at 23°C. The capacity of the three soils to bind DNA (>13 fIg per g of soil)was sufficiently high to adsorb the total DNA of the microbial community living in them. The introduced DNA istributed between the solids and the liquid phase in soil-specific proportions. Kinetic analysis indicated that introduced DNA remaining non-adsorbed was rapidly rendered acid-soluble (within 24 h at 23 0C). In contrast, a large portion of the DNA that bound to the soil particulate material was of high molecular size (up to several kbp)still 5 days after introduction as determined by Southern transfer hybridization. The observations indicate that adsorption of DNA on solid soil components and the concomitant protection against DNases is the mechanism of DNA persistence in soil. Upon hydration of dry soil in the microcosms it was observed that growth of prokaryotes was associated with increased DNase activity in the interstitial soil solution. Suppression of growth by rifampicin and chloramphenicol, but not by cycloheximide, prevented the appearence of DNases. This suggests growing prokaryotes as the main producers of DNases in the soil microcosms. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
SOIL MICROCOSM |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
DNA ADSORPTION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
DNA PERSISTENCE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
MECHANISM |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
EXTRACELLULAR DNA EXTRACTION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
SOIL MICROORGANISMS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
MICROBIAL GROWTH |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
MICROBIAL DNASES |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Blum, S.A.E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Lorenz, M.G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Wackernagel, W. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1gSg80YLKzhlEe5dTDDPagqaNB-e5czqO/view?usp=drivesdk">https://drive.google.com/file/d/1gSg80YLKzhlEe5dTDDPagqaNB-e5czqO/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
