MARC details
| 000 -LEADER |
|---|
| fixed length control field |
01960nam a2200313Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20250625153924.0 |
| 040 ## - CATALOGING SOURCE |
|---|
| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
|---|
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-13506 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Available methods for assembling expression cassettes for synthetic biology |
| 490 0# - SERIES STATEMENT |
|---|
| Volume/sequential designation |
Appl. Microbiol. Biotechnol., 93(5), p.1853-1863, 2012 |
| 520 3# - SUMMARY, ETC. |
|---|
| Summary, etc. |
Studies in the structural biology of the multicomponent protein complex, metabolic engineering, and synthetic biology frequently rely on the efficient over-expression of these subunits or enzymes in the same cell. As a first step, constructing the multiple expression cassettes will be a complicated and time-consuming job if the classic and onventional digestion and ligation based cloning method is used. Some more efficient methods have been developed, including (1)the employment of a multiple compatible plasmid expression system, (2)the rare-cutter-based design of vectors, (3)in vitro recombination (sequence and ligation independent cloning, the isothermally enzymatic assembly of DNA molecules in a single reaction), and (4)in vivo recombination using recombination-efficient yeast (in vivo assembly of overlapping fragments, reiterative recombination for the chromosome integration of foreign expression cassettes). In this review, we systematically introduce these available methods. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
SYNTHETIC BIOLOGY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
SIMULTANEOUS EXPRESSION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
PATHWAY CONSTRUCTION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
YEAST RECOMBINATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
CRE-LOXP SITESPECIFIC |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
RECOMBINATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
ACEMBL SYSTEM |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Wang, T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Ma, X. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Zhu, H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Li, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Du, G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Chen, J. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1iDipvGZwCXAnfZSq2I7vtolbEgKTddh_/view?usp=drivesdk">https://drive.google.com/file/d/1iDipvGZwCXAnfZSq2I7vtolbEgKTddh_/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
