Evidence that Discontinuous DNA Replication in Escherichia coli is Primed by Approximately 10 to 12 Residues of RNA Starting with a Purine (Record no. 47716)

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control field 20250625153924.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-13517
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245 10 - TITLE STATEMENT
Title Evidence that Discontinuous DNA Replication in Escherichia coli is Primed by Approximately 10 to 12 Residues of RNA Starting with a Purine
490 0# - SERIES STATEMENT
Volume/sequential designation J. Mol. Biol., 184(1), p.45-52, 1985
520 3# - SUMMARY, ETC.
Summary, etc. Intact primer RNA for discontinuous DNA replication of Eseheriehiu cohas been detected by specific labeling in vitro of its Fit-terminal tri- (or di-)phosphate group with vaccinia guanylyltransferase and [~z-~*P]GTP. A mutant defective either in RNase H or in bot'h RNase H and DNA polymerase I accumulated about 10 or 30 times more intact primer RNA, respectively, than wild-type cells. The primers started with purine in an A to G rat,io of 5 and the most abundant 5'-terminal dinucleotide sequence was (p)ppA-Pu. The chain length of the intact primer RNA was approximately 10 to 12 nucleotide residues. The structural properties of the E. coli primer RNA resemble those of the eukaryotic primer RNA.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kitanit, T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yoda, K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ogawa, T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Okazaki, T.
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-13517 25.06.2025 25.06.2025 Documentos solicitados