In situ localization of small RNAs in plants by using LNA probes (Record no. 47749)
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| fixed length control field | 01749nam a2200181Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625153924.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-13550 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | In situ localization of small RNAs in plants by using LNA probes |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Nature Protocols, 7, p.533-541, 2012 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Small RNARNARNAs have crucial roles in numerous aspects of plant biology. Despite our current understanding of their biogenesis and mechanisms of action, the biological function of small RNARNARNAs, particularly miRNARNARNAs, remains largely unknown. To decipher small RNARNARNA function, knowledge about their spatiotemporal patterns of expression is essential. Here we report an in situ hybridization method for the precise localization of small RNARNARNAs in plants by using locked nucleic acid (LNALNALNA)oligonucleotide probes. This method has been adapted from protocols used to detect messenger RNARNARNAs in formaldehyde-fixed and paraffin-embedded tissue sections, but it includes essential optimizations in key prehybridization, hybridization and posthybridization steps. Most importantly, optimization of probe concentration and hybridization temperature is required for each unique LNALNALNA probe. We present the detailed protocol starting from sectioned tissues, and we include troubleshooting tips and recommended controls. This method has been used successfully in several plant species and can be completed within 2-6 d. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Javelle, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Timmermans, M.C.P. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1kQcZqhtTYXiibQFc9fNPLPo3mv4guLQD/view?usp=drivesdk">https://drive.google.com/file/d/1kQcZqhtTYXiibQFc9fNPLPo3mv4guLQD/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-13550 | 25.06.2025 | 25.06.2025 | Documentos solicitados |