MARC details
| 000 -LEADER |
|---|
| fixed length control field |
01965nam a2200325Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20250625153925.0 |
| 040 ## - CATALOGING SOURCE |
|---|
| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
|---|
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-13564 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
An efficient and economic enhancer mix for PCR |
| 490 0# - SERIES STATEMENT |
|---|
| Volume/sequential designation |
BioChemical and Biophysical Research Communications, 347(3), p.747-751, 2006 |
| 520 3# - SUMMARY, ETC. |
|---|
| Summary, etc. |
Polymerase chain reaction (PCR)has become a fundamental technique in molecular biology. Nonetheless, further improvements of the existing protocols are required to broaden the applicability of PCR for routine diagnostic purposes, to enhance the specificity and the yield of PCRs as well as to reduce the costs for high-throughput applications. One known problem typically reported in PCR experiments is the poor amplification of GC-rich DNA sequences. Here we designed and tested a novel effective and low-cost PCR enhancer, a concentration- dependent combination of betaine, dithiothreitol, and dimethyl sulfoxide that broadly enhanced the quantitative nd/or qualitative output of PCRs. Additionally, we showed that the performances of this enhancer mix are comparable to those of commercially available PCR additives and highly effective with different DNA polymerases. Thus, we propose the routine application of this PCR enhancer mix for low- and high-throughput experiments. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
POLYMERASE CHAIN REACTION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
GC-RICH SEQUENCE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
ENHANCER |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
ADDITIVE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
PROMOTER PCR |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
GENOMIC PCR |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
PCR TEMPLATE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
TAQ DNA POLYMERASE |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Ralser, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Querfurth, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Warnatz, H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Lehrach, H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Yaspo, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Krobitsch, S. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1G6lgDZEO5Yd39xbV6KCa2lV0Y8tKq8o6/view?usp=drivesdk">https://drive.google.com/file/d/1G6lgDZEO5Yd39xbV6KCa2lV0Y8tKq8o6/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
