Purification of 2-Aminopyridine Derivatives of Oligosaccharides and Related Compounds by Cation-Exchange Chromatography (Record no. 47792)
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| fixed length control field | 02851nam a2200193Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625153925.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-13594 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Purification of 2-Aminopyridine Derivatives of Oligosaccharides and Related Compounds by Cation-Exchange Chromatography |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Analytical BioChemistry, 232(1), p.65-68, 1995 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | A simple method for efficient removal of a large matography (HPLC)on an octadecylsilyl (ODS)column excess of 2-aminopyridine from the reaction mixture and an amide column. This strategy has been recently of pyridylamination of carbohydrates and related extended to include an additional HPLC with a diethylcompounds by cation-exchange chromatography is aminoethyl column for analyses of sialyl-oligosacchapresented. The method is especially suitable for purifi- rides (7). More than 300 neutral and sialyl oligosacchacation and separation of small-sized pyridylaminated- rides have been analyzed by th is approach (5-7), (PA-)derivatives and large-scale preparations. Sepa- attesting to the usefulness of the method. The pyridyration of PA-derivatives from 2-aminopyridine was lamination method has been also used for separation accomplished by a Dowex 50X8 column (NH/4 -form)and purification of oligosaccharides for analyses by eluted with 20 mM ammonium acetate buffer (pH 8.5). NMR and mass spectrometry (8). The PA derivatives thus purified can be used for high- performance liquid chromatography analysis directly. Since more than a 2000-fold excess of 2-aminopyri The method requires only 10 min to purify a sample, in dine (2-AP)is customarily used for the pyridylaminacontrast to an hour or longer required by the gel filtra- tion to obtain quantitative yields (9), removal of the tion method. Unlike the existing methods for purifica- excess reagent from the reaction mixture is an imtion of PA-oligosaccharides, which are applicable only portant step prior to chromatography of the derivatized in the range of 0.05-100 nmol, the cation-exchange products. Gel filtration (5)is effective for removal of method allows processing of much larger quantities of 2-AP from relatively large PA-oligosaccharides, but a PA-derivatives. For example, PA-L-glyceraldehyde (10 complete removal of 2-AP is difficult when small commmol)was successful purified by a Dowex 50X8 column pounds, such as monosaccharides and disaccharides, (1.5 1 17 cm)eluted with 20 mM ammonium acetate are derivatized. Extraction of the reactionmixture with buffer (pH 8.75)within 3 h. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Fan, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Huynh, L.H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lee, Y.C. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1L27nvzg7cRGdqdYkdzQ9hegvCCGss66V/view?usp=drivesdk">https://drive.google.com/file/d/1L27nvzg7cRGdqdYkdzQ9hegvCCGss66V/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-13594 | 25.06.2025 | 25.06.2025 | Documentos solicitados |