The Isolation, Culture and Regeneration of Petunia Leaf Protoplasts' (Record no. 47851)
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| 000 -LEADER | |
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| fixed length control field | 01816nam a2200193Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625153926.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-13653 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | The Isolation, Culture and Regeneration of Petunia Leaf Protoplasts' |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Developmental Biology, 33, p.130-137, 1973 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Methods are described for the enzymatic release of protoplasts from leaves of Petunia hybrida and for the utilization of protoplasts in studies in plant developmental biology. As a result of spontaneous fusion during cell wall degradation of leaf material, fresh preparations can contain a high proportion of multinucleate protoplasts. This level can be dramatically reduced by a gradual plasmolysis of the material prior to enzyme incubation. Leaf protoplasts maintained in liquid media are seen to undergo cell wall synthesis, "budding," and limited regenerated cell division sometimes associated with anthocyanin production. Under such conditions, multinucleate cells are formed as a result of mitosis without cytokinesis. Protoplasts, plated out in a fully defined medium, undergo cell wall synthesis followed by sustained progeny cell division with eventual cell colony production. Cell colonies, derived from individual mesophyll protoplasts, grow rapidly upon subculture, to produce callus capable of shoot differentiation d ultimately whole plant formation. Protoplasts isolated from varieties of P. hybrida were found to differ in their cultural requirements. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Frearson, E.M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Power, J.B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Cocking, E.C. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/19s6m8GgGz3Lc9OG28VsXiJTzFL2UdiSs/view?usp=drivesdk">https://drive.google.com/file/d/19s6m8GgGz3Lc9OG28VsXiJTzFL2UdiSs/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-13653 | 25.06.2025 | 25.06.2025 | Documentos solicitados |