A New Method of Sequencing DNA (Record no. 47888)

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control field MX-MdCICY
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control field 20250625153927.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-13690
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Title A New Method of Sequencing DNA
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Volume/sequential designation Analytical BioChemistry, 174(2), p.423-436, 1988
520 3# - SUMMARY, ETC.
Summary, etc. An entirely new method of sequencing DNA has been devised that does not use electrophoresis, radioactivity, or fluorescence. The method works by measuring pyrophosphate generated by the DNA polymerization reaction. DNA and DNA polymerase are held by a DEAE-Sepharose column and solutions containing different dNTPs are pumped through. The pyrophosphate generated is measured continuously by a device consisting of a series of columns containing enzymes covalently attached to Sepharose. The alternating copolymer poly(dA.dT)is sequenced as an illustration of the method. Future improvements that will facilitate automation are discussed
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DNA SEQUENCING
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Topical term or geographic name entry element LUCIFERASE
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Topical term or geographic name entry element ATP SULFURYLASE
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Topical term or geographic name entry element PYROPHOSPHATE
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Topical term or geographic name entry element SEPHAROSE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DNA POLYMERASE
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Personal name Hyman, E.D.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1Eh5kgsBKTKJBv52CtiHprY0X1K81zpZy/view?usp=drivesdk">https://drive.google.com/file/d/1Eh5kgsBKTKJBv52CtiHprY0X1K81zpZy/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-13690 25.06.2025 25.06.2025 Documentos solicitados