Enzymatic Method for Continuous Monitoring of Inorganic Pyrophosphate Synthesis (Record no. 47889)

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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-13691
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Title Enzymatic Method for Continuous Monitoring of Inorganic Pyrophosphate Synthesis
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Volume/sequential designation Analytical BioChemistry, 151(2), p.504-509, 1985
520 3# - SUMMARY, ETC.
Summary, etc. A sensitive method for the analysis of inorganic pyrophosphate (PP,)which utilizes the enzymes ATP sulfurylase and firefly luciferase is described. The assay is based on continuous monitoring of the ATP formed in the ATP sulfurylase reaction using purified firefly luciferase. The assay can be completed in less than 2 s and is not affected by inorganic phosphate. The method has been used for continuous monitoring of formation Of PPi in Rhodospirillum rubrum chromatophores. The assay is extremely sensitive, the linear range of the assay being I . 10e9-5. IO-' M PPi. It is suitable for routine applications. It is also possible to use the method for determination of low amounts of adenosine S'phosphosulfate.
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Topical term or geographic name entry element LUMINESCENCE
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Topical term or geographic name entry element INORGANIC ANALYSIS
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Topical term or geographic name entry element PHOSPHATE COMPOUNDS
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Topical term or geographic name entry element MEMBRANE-BOUND ENZYMES
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Topical term or geographic name entry element PHOTOSYNTHESIS
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Topical term or geographic name entry element BIOENERGETICS
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Personal name Nyren, P.
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Personal name Lundin, A.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/1ucE-k-lreMJ6QShqjxa15LDLNAtHeRgA/view?usp=drivesdk">https://drive.google.com/file/d/1ucE-k-lreMJ6QShqjxa15LDLNAtHeRgA/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-13691 25.06.2025 25.06.2025 Documentos solicitados