MARC details
| 000 -LEADER |
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02129nam a2200229Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625153927.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-13704 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
An Escherichia coli Cell Membrane Chromatography-Offline LC-TOF-MS Method for Screening and Identifying Antimicrobial Peptides from Jatropha curcas Meal Protein Isolate Hydrolysates |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Journal of BioMolecular Screening, DOI: 10.1177/1087057112442744, 2012 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
A novel, simple, and rapid method, named cell membrane affinity extraction (CMAE)-offline liquid chromatography timeof-flight mass spectrometry (LC-TOF-MS)was developed for screening and identifying antimicrobial peptides from Jatropha curcas meal protein isolate hydrolysates (JCMPIH)obtained by proteolytic enzyme (pepsin, psin, protamex, neutrase, flavourzyme, papain, alcalase, and acid protease)hydrolysis. A cationic antimicrobial peptide (CAILTHKR, JCpep8)was successfully isolated and identified by this method. Antimicrobial assay indicated that JCpep8 was active against the tested microorganisms (Escherichia coli ATCC 25922, Shigella dysenteriae ATCC 51302, Pseudomonas aeruginosa ATCC 27553, Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 23631, Streptococcus pneumoniae ATCC 49619)with minimal inhibitory concentration values ranging from 29 to 68 ìg/mL. JCpep8 induced significant morphological alterations of the tested microbe surfaces, as shown by transmission electron microscopy, indicating strong membrane disruption. The results showed that CMAE-offline LC-TOF-MS could be a promising method for discovering high-throughput screening antimicrobial peptides from JCMPIH. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CELL MEMBRANE AFFINITY EXTRACTION (CMAE) |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
HIGH-THROUGHPUT SCREENING (HTS) |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ANTIMICROBIAL PEPTIDES |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
JATROPHA CURCAS |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Xiao, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Zhang, H. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1nLUQoEzkZnu6NdWyLgcdnbvjyLSsRV4h/view?usp=drivesdk">https://drive.google.com/file/d/1nLUQoEzkZnu6NdWyLgcdnbvjyLSsRV4h/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
