Microbial responses to xenobiotic compounds. Identification of genes that allow Pseudomonas putida KT2440 to cope with 2,4,6-trinitrotoluene (Record no. 47916)
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| fixed length control field | 02005nam a2200229Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625153928.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-13718 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Microbial responses to xenobiotic compounds. Identification of genes that allow Pseudomonas putida KT2440 to cope with 2,4,6-trinitrotoluene |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Microbial Biotechnology, 2(2), p.287-294, 2009 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Pseudomonas putida KT2440 grows in M9 minimal medium with glucose in the presence of 2,4,6- trinitrotoluene (TNT)at a similar rate than in the absence of TNT, although global transcriptional analysis using DNA microarrays revealed that TNT exerts some stress. Response to TNT stress is regulated at the transcriptional level, as significant changes in the level of expression of 65 genes were observed. Of these genes, 39 appeared upregulated, and 26 were downregulated. The identity of upregulated genes suggests that P. putida uses two kinds of strategies to overcome TNT toxicity: (i)induction of genes encoding nitroreductases and detoxification-related enzymes (pnrA, xenD, acpD)and (ii)induction of multidrug efflux pump genes (mexEF/oprN)to reduce intracellular TNT concentrations. Mutants of 13 upand 7 downregulated genes were analysed with regards to TNT toxicity revealing the role of the MexE/MexF/OprN pump and a putative isoquinoline 1-oxidoreductase in tolerance to TNT. TheORFPP1232 whose transcriptional level did not change in response to TNT affected growth in the presence of nitroaromatic compounds and it was found in a screening of 4000 randomly generated mutants. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Fernández, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Duque, E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Pizarro-Tobías, P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Van Dillewijn, P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wittich, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ramos, J.L. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1d7MgslwfbDx6WnQOx1ALyfXJEPWjjiOU/view?usp=drivesdk">https://drive.google.com/file/d/1d7MgslwfbDx6WnQOx1ALyfXJEPWjjiOU/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-13718 | 25.06.2025 | 25.06.2025 | Documentos solicitados |