Aspergillus niger lipases: induction, isolation and characterization of two lipases from a MZKI Al 16 strain (Record no. 47961)

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fixed length control field 01780nam a2200229Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625153928.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-13763
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245 10 - TITLE STATEMENT
Title Aspergillus niger lipases: induction, isolation and characterization of two lipases from a MZKI Al 16 strain
490 0# - SERIES STATEMENT
Volume/sequential designation Journal of Molecular Catalysis B: Enzymatic, 2, p.215-222, 1997
520 3# - SUMMARY, ETC.
Summary, etc. Aspergillus niger strain MZKI Al 16 was used to produce lipolytic enzymes in submerged culture. Lipase production was induced by addition of olive oil to a complex medium with an initial pH of 5.0. Maximal activity was reached after 70 h in a 15 1 bioreactor at 30°C with aeration of 0.5 vvm and agitation 400 rpm. Optimal temperature and pH conditions for the action of the lipases tested on tributyrin were 45°C and pH 7.0. Triacetin and tributyrin were shown to be the best substrates. The presence of iron and silver ions at low concentrations did not alter the activities. Two enzymes possessing lipase activity were isolated by acetone precipitation followed by ion-exchange chromatography. The molecular weights were 43 kDa and 65 kDa with isoelectric points of pH 4.1 and 4.2, respectively. The higher molecular weight lipase showed preference toward 1- and 3-positions of the triglyceride molecule and was stereoselective for the sn- 1 position with an enantiomeric excess of 20
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element LIPASES
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ASPERGILLUS NIGER
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SELECTIVITY
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Pokorny, D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Cimerman, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Steiner, W.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1JU79kQLMv9NuddfCaAJ6K2npB3RLzIMW/view?usp=drivesdk">https://drive.google.com/file/d/1JU79kQLMv9NuddfCaAJ6K2npB3RLzIMW/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-13763 25.06.2025 25.06.2025 Documentos solicitados