MARC details
| 000 -LEADER |
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| fixed length control field |
02155nam a2200253Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625153931.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-13928 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Auxin-regulated gene expression in intact soybean hypocotyl and excised hypocotyl sections |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Planta, 162(2), p.147-153, 1984 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
A library of complementary DNA (cDNA)clones has been prepared from polyadenylated RNA (poly(A)+RNA)from auxin (2,4-dichlorophenoxyacetic acid)-treated soybean (Glycine max (L.)Merr. cv. Wayne)seedlings. Using differential hybridization, four clones were selected as auxin-responsive, and characterized. The levels of the RNA sequences homologous to the cDNA clones were examined in the hypocotyl of the intact seedling and in excised hypocotyl sections before and after auxin treatment, using RNA blot hybridization analysis. RNA levels are rapidly increased (within 0.25-0.5 h)following auxin treatment and the response in the hypocotyl of the intact seedling is transient, reaching maximum RNA levels 2-4 h after auxin application. Increases in RNA levels were also observed with the auxins indole 3-acetic acid and 2,4,5-trichlorophenoxyacetic acid, but not with the ethylene-producing compound, Ethephon (2-chloroethylphosphonic acid). Hybridization analysis of in-vitro transcription products made in nuclei isolated from untreated and auxin-treated soybean primary leaves and excised hypocotyl sections indicates that, for the two cDNA clones analyzed, the increased RNA levels in auxin-treated organs are at least partially the result of increased transcriptional activity of specific DNA sequences. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
AUXIN INDUCED MRNA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CDNA CLONES |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
GLYCINE (AUXIN AND GENE EXPRESSION) |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
NUCLEAR "RUN OFF" TRANSCRIPTION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
RNA BLOTS. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Hagen, G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Kleinschmidt, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Guilfoyle, T. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1TecWsue52oHtuTLNjpXbmLdQPskEKr-p/view?usp=drivesdk">https://drive.google.com/file/d/1TecWsue52oHtuTLNjpXbmLdQPskEKr-p/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
