MARC details
| 000 -LEADER |
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| fixed length control field |
01715nam a2200241Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625153932.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-13930 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Determination of indole-3-pyruvic acid levels in Arabidopsis thaliana by gas chromatography-selected ion monitoring-mass spectrometry |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Journal of Chromatography A, 800(1), p.101-108, 1998 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
A rapid and simple method is described for the determination of indole-3-pyruvic acid (IPA)levels in Arabidopsis thaliana by gas chromatography-selected ion monitoring-mass spectrometry (GC-SIM-MS). The method includes derivatization of IPA with hydroxylamine in the crude extract, followed by ethyl acetate partitioning, solid-phase extraction with C resin, reversed-phase high-performance liquid chromatography (HPLC), and GC-SIM-MS. Three derivatizing 18 reagents were tested; these were entafluorobenzylhydroxylamine, pentafluorophenylhydrazine, and hydroxylamine. Hydroxylamine proved to be the most useful, as the IPA-oxime was easiest to purify from plant extracts and was the most stable. IPA was quantified in Arabidopsis seedlings ranging in age from 5 to 12 days; levels varied from 4 to 13 ng/ g, peaking at 7-9 days. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ARABIDOPSIS THALIANA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
DERIVATIZATION, GC |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
INDOLEPYRUVIC ACID |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PENTAFLUOROPHENYLHYDRAZINE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PENTAFLUOROBENZYLHYDROXYLAMINE |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Tam, Y.Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Normanly, J. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1P0PNyPuM9oprfio3F9dBIN_BNzb2hKd3/view?usp=drivesdk">https://drive.google.com/file/d/1P0PNyPuM9oprfio3F9dBIN_BNzb2hKd3/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
