Natural variation in rice starch synthase iia affects enzyme and starch properties (Record no. 48138)

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003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625153932.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-13941
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245 10 - TITLE STATEMENT
Title Natural variation in rice starch synthase iia affects enzyme and starch properties
490 0# - SERIES STATEMENT
Volume/sequential designation Functional Plant Biology, 31(7), p.671-684, 2004
520 3# - SUMMARY, ETC.
Summary, etc. The natural variation in starch synthase IIa (SSIIa)of rice (Oryza sativa L.)was characterised using near-isogenic lines (NILs). SSIIa is a candidate for the alk gene regulating the alkali disintegration of rice grains, since both genes are genetically mapped at the same position on chromosome 6 and related to starch properties. In this study, we report that the alkali-susceptible cultivar Nipponbare lacked SSIIa activity in endosperm. However, the activity was detected with NILs having the alk allele of alkali-tolerant Kasalath. SSIIa protein was present even in Nipponbare endosperm, but it was not associated with starch granules at the milky stage of endosperm. Three single-nucleotide polymorphisms (SNPs)predicting amino acid substitutions existed between the cDNA sequences of SSIIa of Nipponbare and Kasalath were genotyped with 65 rice cultivars and four wild relatives of cultivated rice. The results obtained explain the potential importance of two of the amino acid residues for starch association of rice SSIIa. An analysis of the chain-length distribution of ß-limit dextrin of amylopectin showed that without SSIIa activity, the relative number of A-chains (the short chains without branches)increased and that of B1-chains (the short chains with branches)decreased. This suggests that, given the SSIIa defect, short A-chains could not reach a sufficient length for branching enzymes to act on them to produce B1-chains
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ALK
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ALKALI DISINTEGRATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element AMYLOPECTIN
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element RICE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element STARCH SYNTHASE
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Umemoto, T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Aoki, N.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Lin, H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Nakamura, Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Inouchi, N.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sato, Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yano, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yano, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Maruyama ,S.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/14Bmh8PQgn74-lvWMWO3T2e634iUoWCrv/view?usp=drivesdk">https://drive.google.com/file/d/14Bmh8PQgn74-lvWMWO3T2e634iUoWCrv/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-13941 25.06.2025 25.06.2025 Documentos solicitados