An improved technique for the efficient construction of gene libraries by partial filling-in of cohesive ends (Record no. 48252)
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| fixed length control field | 01563nam a2200181Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625153934.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-14057 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | An improved technique for the efficient construction of gene libraries by partial filling-in of cohesive ends |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Gene, 42(1), p.119-123, 1986 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | For the preparation of gene libraries, DNA from iEMBL3 phage was digested with Sal1 and EcoRI, and the cohesive ends partially filled-in by addition of dTTP, dCTP and Klenow fragment of DNA polymerase I (PolIk). Genomic DNA was cleaved partially with Sau3A and subsequently incubated with dATP, dGTP and PolIk. The phage and genomic DNAs were then mixed and ligated. The recombinant DNAs were packaged in vitro. The efficiency of packaging was 105-lo6 of infectious phage 1, particles per pg of the genomic DNA (as compared to approx. 10' per pg for the wild-type 2 DNA). This procedure is very rapid and requires only pg quantities of genomic DNA for preparing an entire gene library. The other important advantage is that multiple independent insertions of genomic DNA cannot occur in a single recombinant phage and self-ligation of phage DNA is blocked. It is also applicable for other z/I-containing vectors. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zabarovsky, E.R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Allikmets, R.L. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1-kFY1WiwXAyBudu3XZmzKXRcw9jxzGAY/view?usp=drivesdk">https://drive.google.com/file/d/1-kFY1WiwXAyBudu3XZmzKXRcw9jxzGAY/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-14057 | 25.06.2025 | 25.06.2025 | Documentos solicitados |