MAP Kinase Phosphorylation and cAMP Assessment in Fungi (Record no. 48443)

MARC details
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fixed length control field 01847nam a2200313Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625153938.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-14249
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Title MAP Kinase Phosphorylation and cAMP Assessment in Fungi
490 0# - SERIES STATEMENT
Volume/sequential designation Methods in Molecular Biology, 835, p.571-583, 2012
520 3# - SUMMARY, ETC.
Summary, etc. The cyclic AMP (cAMP)signaling and mitogen-activated protein (MAP)kinase pathways are the most important signal transduction pathways in eukaryotes. In many plant pathogenic fungi they play pivotal roles in virulence and development. Identifi cation and understanding the role of signal transduction pathways in regulation of cellular responses require robust biochemical techniques. Determination of both the phosphorylation status of MAPKs and the intracellular levels of cAMP is required to unravel the function of these pathways during adaptation of fungi to environmental stress conditions or when particular fungal genes are disrupted or silenced. Here we describe protocols to determine the phosphorylation status of three different MAPKs including Fus3, Slt2 and Hog1 as well as a protocol to measure the intracellular levels of cAMP levels. These protocols can be adapted for a wide range of fungi.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CAMP MEASUREMENT
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MITOGEN-ACTIVATED PROTEIN KINASE
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Topical term or geographic name entry element PROTEIN KINASE A
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Topical term or geographic name entry element PHOSPHORYLATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element FUS3
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SLT2
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element HOG1
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Mehrabi, R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name M'Barek, S.B.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Saidi, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Abrinbana, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name De Wit, P.J.G.M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kema, G.H.J.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1hDFDavhdoRGdZNfxSSGrj-6dLeI28E3z/view?usp=drivesdk">https://drive.google.com/file/d/1hDFDavhdoRGdZNfxSSGrj-6dLeI28E3z/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-14249 25.06.2025 25.06.2025 Documentos solicitados