Multiplex RT-PCR detection of four aphid-borne strawberry viruses in Fragaria spp. in combination with a plant mRNA specific internal control (Record no. 48542)
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| fixed length control field | 02089nam a2200241Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625153939.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-14349 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Multiplex RT-PCR detection of four aphid-borne strawberry viruses in Fragaria spp. in combination with a plant mRNA specific internal control |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Journal of Virological Methods, 111, p.85-93, 2003 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The principal aphid-borne viruses infecting Strawberry (Fragaria spp.)Strawberry crinkle virus (SCV), Strawberry mild yellow edge virus (SMYEV), Strawberry mottle virus (SMoV)and Strawberry vein banding virus (SVBV)can cause serious crop losses. In this paper, a multiplex reverse transcriptase polymerase chain reaction (RT-PCR)method is described for the simultaneous detection of all four viruses in combination with a plant mRNA specific internal control which can be used as an indicator of the effectiveness of the extraction and RT-PCR. In total, 18 strawberry isolates infected naturally were analysed by this method. Every combination of RNA virus was able to be detected and a full complement of all four viruses were found together in three isolates, all taken from wild strawberry (Fragaria chiloensis (L.)Duch.)in Chile. The upper detection limit for the four viruses was at an extract dilution of 1/200. The broad applicability of the RNA specific internal control primers*/which produced a PCR fragment of the expected size in 25 of 27 plant species tested*/combined with improvements, made in extraction methods described provides potentially a standard method for comparable RT-PCR analyses in a wide variety of plant species. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | SILICA CAPTURE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | NDHB GENE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ATROPA BELLADONNA |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Thompson, J.R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wetzel, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Klerks, M.M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Vaskova, D. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1y8qInRGn5lbx9lZch3bj4jSW2R_CfVXx/view?usp=drivesdk">https://drive.google.com/file/d/1y8qInRGn5lbx9lZch3bj4jSW2R_CfVXx/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-14349 | 25.06.2025 | 25.06.2025 | Documentos solicitados |