Secretome of the Free-living Mycelium from the Ectomycorrhizal Basidiomycete Laccaria bicolor (Record no. 48559)

MARC details
000 -LEADER
fixed length control field 02448nam a2200337Ia 4500
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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625153940.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-14366
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245 10 - TITLE STATEMENT
Title Secretome of the Free-living Mycelium from the Ectomycorrhizal Basidiomycete Laccaria bicolor
490 0# - SERIES STATEMENT
Volume/sequential designation J. Proteome Res, 11(1), p.157-171, 2011
520 3# - SUMMARY, ETC.
Summary, etc. The ectomycorrhizal basidiomycete Laccaria bicolor has a dual lifestyle with a transitory soil saprotrophic phase and a longer mutualistic interaction with tree roots. Recent evidence suggests that secreted proteins play key roles in host plant colonisation and symbiosis development. However, a limited number of secreted proteins have been characterized, and the full spectrum of effectors involved in the mycobiont invasion and survival remains unknown. We analyzed the extracellular proteins secreted in growth medium by free-living mycelium of L. bicolor as a proxy for its saprotrophic phase. The proteomic analyses (two-dimensional electrophoresis and shotgun proteomics)were substantiated by wholegenome expression transcript profiling on ectomycorrhizal roots. Among the 224 proteins identified were carbohydrate-acting enzymes likely involved in the cell wall remodelling linked to hyphal growth as well as secreted proteases possibly digesting soil organic compounds and/or fending off competitors, pathogens, and predators. Evidence of gene expression was found in ectomycorrhizal roots for 210 of them. These findings provide the first global view of the secretome of a mutualistic symbiont and shed some light on the mechanisms controlling cell wall remodelling during the hyphal growth. They also revealed many novel putative secreted proteins of unknown function, including one mycorrhiza-induced small secreted protein.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element 2-DE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ECTOMYCORRHIZAL FUNGUS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element LACCARIA BICOLOR
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Topical term or geographic name entry element MYCELIUM
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SECRETED PROTEINS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SECRETOMIC
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SHOTGUN PROTEOMICS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SYMBIOSIS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Vincent, D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kohler, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Claverol, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Solier, E.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Joets, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Gibon, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Lebrun, M-H
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1MJPpOuXMh4-t2qR5f_BC-HXUOJm9I4Ui/view?usp=drivesdk">https://drive.google.com/file/d/1MJPpOuXMh4-t2qR5f_BC-HXUOJm9I4Ui/view?usp=drivesdk</a>
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942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-14366 25.06.2025 25.06.2025 Documentos solicitados