Discovery and Functional Analysis of Monoterpenoid Indole Alkaloid Pathways in Plants (Record no. 48560)
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| fixed length control field | 02461nam a2200193Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625153940.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-14368 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Discovery and Functional Analysis of Monoterpenoid Indole Alkaloid Pathways in Plants |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Methods in Enzymology, 515, p.207-229, 2012 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Numerous difficulties have been associated with forward genetic approaches to identify, and functionally characterize genes involved in the biosynthesis, regulation, and transport of monoterpenoid indole alkaloids (MIAs). While the identification of certain classes of genes associated with MIA pathways has facilitated the use of homology-based approaches to clone other genes catalyzing similar reactions in other parts of the pathway, this has not greatly speeded up the pace of gene discovery for the diversity of reactions involved. Compounding this problem has been the lack of knowledge or even availability of certain MIA intermediates that would be required to establish a novel enzyme reaction to functionally identify a biosynthetic step or the candidate gene product involved. The advent of inexpensive sequencing technologies for transcriptome and genome sequencing, combined with proteomics and metabolomics, is now revolutionizing the pace of gene discovery associated with MIA pathways and their regulation. The discovery process uses large databases of genes, proteins, and metabolites from an everexpanding list of nonmodel plant species competent to produce and accumulate MIAs. Comparative bioinformatics between species, together with gene expression analysis of particular tissue, cell, and developmental types, is helping to identify target genes that can then be investigated for their possible role in an MIA pathway by virus-induced gene silencing. Successful silencing not only confirms the involvement of the candidate gene but also allows identification of the pathway intermediate involved. In many circumstances, the pathway intermediate can be isolated for use as a substrate in order to confirm gene function in heterologous bacterial, yeast, or plant expression systems. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | De Luca, V. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Salim, V. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Levac, D. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1lnnNORhlS0bD7oH0Ybqh46oHzZfvFLT5/view?usp=drivesdk">https://drive.google.com/file/d/1lnnNORhlS0bD7oH0Ybqh46oHzZfvFLT5/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-14368 | 25.06.2025 | 25.06.2025 | Documentos solicitados |