MARC details
| 000 -LEADER |
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| fixed length control field |
02157nam a2200241Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625153943.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-14548 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Growth factors and fibrochondrocytes in scaffolds |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Journal of Orthopaedic Research, 23(5), p.1184-1190, 2005 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Four growth factors, transforming growth factor-pl (TGF-PI), platelet-derived growth factor-AB (PDGF-AB), insulin-like growth factor I (IGF-I), and basic fibroblastic growth factor (bFGF), were tested at different concentrations for their effects on extracellular matrix (ECM)production in three-dimensional cultures of meniscal fibrochondrocytes. Cells from New Zealand white rabbits were seeded on poly-glycolic acid (PGA)scaffolds and were stimulated with growth factors for three weeks. 3H-proline and 35S-sulfate labels were used to measure uptake of collagen and glycosaminoglycan (GAG)components, respectively. Biochemical assays were performed to measure the total collagen, GAG, and DNA present in the scaffolds at the end of the study. TGF-PI (10 and 100 ng/ml)stimulated both 3H-proline and 3SS-sulfateu ptake, showing a dose-dependent response for both and a temporal response for 35S-sulfate uptake. IGF-I (5 ng/ml)and bFGF (25 and 100 ng/ml)showed increases in 3H-proline uptake by the third week of growth factor addition. PDGF-AB did not show notable increases in uptake. Because TGF-p1 (10 and 100 ng/ml)had visibly denser scaffolds, as evidenced by gross microscopy, at lOOx, and the strongest uptake responses to both "S-sulfate and 3H-proline, it appears to be the most effective growth factor for use in scaffold-based approaches to tissue engineer the knee meniscus. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
MENISCUS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
FIBROCHONDROCYTES |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
GROWTH FACTORS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
COLLAGEN |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
GLYCOSAMINOGLYCANS |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Pangborn, C.A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Athanasiou, K.A. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1W-xVeGGDKkIHIAUOVpqZEWPIoyzURmUe/view?usp=drivesdk">https://drive.google.com/file/d/1W-xVeGGDKkIHIAUOVpqZEWPIoyzURmUe/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
