Restriction fragment length polymorphism of ribomal internal transcribed spacer and 5.8s regions in japanes Alexandrium species (dinophyceae) (Record no. 48762)

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control field MX-MdCICY
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control field 20250625153944.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-14575
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245 10 - TITLE STATEMENT
Title Restriction fragment length polymorphism of ribomal internal transcribed spacer and 5.8s regions in japanes Alexandrium species (dinophyceae)
490 0# - SERIES STATEMENT
Volume/sequential designation Journal Phycology, 30, p.857-863, 1994
520 3# - SUMMARY, ETC.
Summary, etc. The 5.8S ribosomal RNA (rDNA)gene and flanking internal transcribed spacers (ITS1 and ITS2)from 9 isolates of Alexandrium catenella (Whedon and Kofoid)Taylor, 11 isolates of A. tamarense (Lebour)Taylor, and single isolates of A. affine (Inoue et Fukuyo)Balech, A. insuetum Balech, and A. pseudogonyaulax (Biecheler)Horiguchi ex Yuki et Fukuyo comb. nov. from various locations in Japan were amplified using the polymerase chain reaction (PCR)and subjected to restriction fragment-length polymorphism (RFLP)analysis. PCR products from all strains were approximately 610 bp, inclusive of a limited region of the 18S and 28S rRNA coding regions. RFLP analysis using four restriction enzymes revealed six distinct classes of rDNA ("ITS types"). Restriction patterns of A. catenella were uniform at the intra-specific level and clearly distinguishable from those of A. tamarense. The patterns associated with A. tamarense ("tamarense group")were also uniform except for one strain, WKS-1. Some restriction fragments from WKS-1 were in common with those of A. catenella or A. tamarense, whereas some were distinct from all Alexandrium species tested. Alexandrium affine, A. insuetum, and A. pseudogonyaulax carry unique ITS types. The ITSs of the "tamarense group" exhibit sequence heterogeneity. In contrast, the ITSs of all other isolates (including WKS-1)appear homogeneous. RFLP analysis of the 5.8S rDNA and flanking ITSs regions from Alexandrium species reveals useful taxonomic and genetic markers at the species and/or population levels.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ALEXANDRIUM CATENELLA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ALEXANDRIUM TAMARENSE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DINOPHYCEAE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element INTERNAL TRANSCRIBED SPACER
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MOLECULAR IDENTIFICATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element RIBOSOMAL DNA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element RFLP
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TOXIC DINOFLAGELLATES
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Adachi, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yoshihiko, S
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yuzaburo, I.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1HS1UeK6hmp7SzIzheDCBYVjAJsqJdBS3/view?usp=drivesdk">https://drive.google.com/file/d/1HS1UeK6hmp7SzIzheDCBYVjAJsqJdBS3/view?usp=drivesdk</a>
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