Molecular characterization of cyanobacterial diversity in a shallow eutrophic lake (Record no. 48806)

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control field MX-MdCICY
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control field 20250625153945.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-14620
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Title Molecular characterization of cyanobacterial diversity in a shallow eutrophic lake
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Volume/sequential designation Environmental MicroBiology, 7, p.365-377, 2005
520 3# - SUMMARY, ETC.
Summary, etc. We have studied the diversity of pelagic cyanobacteria in Lake Loosdrecht, the Netherlands, through recovery and analysis of small subunit ribosomal RNA gene sequences from lake samples and cyanobacterial isolates. We used an adapted protocol for specific amplification of cyanobacterial rDNA for denaturing gradient gel electrophoresis (DGGE)analysis. This protocol enabled direct comparison of cyanobacterial community profiles with overall bacterial profiles. The theoretical amplification specificity of the primers was supported by sequence analysis of DNA from excised DGGE bands. Sequences recovered from these bands, in addition to sequences obtained by polymerase chain reaction (PCR)and cloning from lake DNA as well as from cyanobacterial isolates from the lake, revealed a diverse consortium of cyanobacteria, among which are representatives of the genera Aphanizomenon , Planktothrix , Microcystis and Synechococcus . One numerically important and persistent cyanobacterium in the lake, Prochlorothrix hollandica , appeared to co-occur with an unknown but related species. However, the lake is dominated by filamentous species that originally have been termed ' Oscillatoria limneticalike'. We show that this is a group of several related cyanobacteria, cooccurring in the lake, which belong to the Limnothrix / Pseudanabaena group. The available variation among the coexisting strains of this group can explain the persistent dominance of the group under severe viral pressure.
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Personal name Zwart, G.
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Personal name Kamst-Van Aggterveld, M.P:
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Personal name Van Der Werff-Staverman, I.
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Personal name Hagen, F.
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Personal name Hoogveld, H.L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Gons, H.J.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/1ujvrVfzG1-_hzWTWcZIT-fbcofBKGXcE/view?usp=drivesdk">https://drive.google.com/file/d/1ujvrVfzG1-_hzWTWcZIT-fbcofBKGXcE/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-14620 25.06.2025 25.06.2025 Documentos solicitados