A comparative molecular analysis of water-filled limestone sinkholes in north-eastern Mexico (Record no. 48819)

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control field MX-MdCICY
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control field 20250625153945.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-14633
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Title A comparative molecular analysis of water-filled limestone sinkholes in north-eastern Mexico
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Volume/sequential designation Environmental MicroBiology, 13(1), p.226-240, 2010
520 3# - SUMMARY, ETC.
Summary, etc. Sistema Zacatón in north-eastern Mexico is host to several deep, water-filled, anoxic, karstic sinkholes (cenotes). These cenotes were explored, mapped, and geochemically and microbiologically sampled by the autonomous underwater vehicle deep phreatic thermal explorer (DEPTHX). The community structure of the filterable fraction of the water column and extensive microbial mats that coat the cenote walls was investigated by comparative analysis of smallsubunit (SSU)16S rRNA gene sequences. Full-length Sanger gene sequence analysis revealed novel microbial diversity that included three putative bacterial candidate phyla and three additional groups that showed high intra-clade distance with poorly characterized bacterial candidate phyla. Limited functional gene sequence analysis in these anoxic environments identified genes associated with methanogenesis, sulfate reduction and anaerobic ammonium oxidation. A directed, barcoded amplicon, multiplex pyrosequencing approach was employed to compare 100 000 bacterial SSU gene sequences from water column and wall microbial mat samples from five cenotes in Sistema Zacatón. A new, high-resolution sequence distribution profile (SDP)method identified changes in specific phylogenetic types (phylotypes)in microbial mats at varied depths; Mantel tests showed a correlation of the genetic distances between mat communities in two cenotes and the geographic location of each cenote. Community structure profiles from the water column of three neighbouring cenotes showed distinct variation; statistically significant differences in the concentration of geochemical constituents suggest that the variation observed in microbial communities between neighbouring cenotes are due to geochemical variation.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sahl, J.W.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Gary, M.O.
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Personal name Harris, J.K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Spear, J.R.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1WeFoL8dblW5egn9WnrWcddBdFndr5qiY/view?usp=drivesdk">https://drive.google.com/file/d/1WeFoL8dblW5egn9WnrWcddBdFndr5qiY/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-14633 25.06.2025 25.06.2025 Documentos solicitados