Analysis of Secreted Proteins (Record no. 48877)

MARC details
000 -LEADER
fixed length control field 02456nam a2200253Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625153946.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-14692
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
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245 10 - TITLE STATEMENT
Title Analysis of Secreted Proteins
490 0# - SERIES STATEMENT
Volume/sequential designation Proteomics for Biomarker Discovery, p.37-60, 2013
520 3# - SUMMARY, ETC.
Summary, etc. Most biological processes including growth, proliferation, differentiation, and apoptosis are coordinated by tightly regulated signaling pathways, which also involve secreted proteins acting in an autocrine and/ or paracrine manner. In addition, extracellular signaling molecules affect local niche biology and in fl uence the cross-talking with the surrounding tissues. The understanding of this molecular language may provide an integrated and broader view of cellular regulatory networks under physiological and pathological conditions. In this context, the pro fi ling at a global level of cell secretomes (i.e., the subpopulations of a proteome secreted by the cell)has become an active area of research. The current interest in secretome research also deals with its high potential for the biomarker discovery and the identi fi cation of new targets for therapeutic strategies. Several proteomic and mass spectrometry platforms and methodologies have been applied to secretome pro fi ling of conditioned media of cultured cell lines and primary cells. Nevertheless, the analysis of secreted proteins is still a very challenging task, because of the technical dif fi culties that may hamper the subsequent mass spectrometry analysis. This chapter describes a typical work fl ow for the analysis of proteins secreted by cultured cells. Crucial issues related to cell culture conditions for the collection of conditioned media, secretome preparation, and mass spectrometry analysis are discussed. Furthermore, an overview of quantitative LC-MS-based approaches, computational tools for data analysis, and strategies for validation of potential secretome biomarkers is also presented.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SECRETOME
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PROTEOMICS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BIOMARKER DISCOVERY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MASS SPECTROMETRY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element QUANTITATIVE LC-MS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Severino, V.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Farina, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Chambery, A.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1vwiT9gzYOf_3_HfCDfOVl4uSO9vai_w_/view?usp=drivesdk">https://drive.google.com/file/d/1vwiT9gzYOf_3_HfCDfOVl4uSO9vai_w_/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-14692 25.06.2025 25.06.2025 Documentos solicitados