Global Identi fi cation of Small RNA Targets in Plants by Sequencing Sliced Ends of Messenger RNAs (Record no. 48880)

MARC details
000 -LEADER
fixed length control field 01492nam a2200229Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625153946.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-14695
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245 10 - TITLE STATEMENT
Title Global Identi fi cation of Small RNA Targets in Plants by Sequencing Sliced Ends of Messenger RNAs
490 0# - SERIES STATEMENT
Volume/sequential designation Rice Protocols, 119-129, 2013
520 3# - SUMMARY, ETC.
Summary, etc. Small RNAs (microRNAs and other classes of endogenous small interfering RNAs)play important roles in a wide variety of biological processes. However, integration of small RNAs in diverse biological networks relies on the con fi rmation of their RNA targets. In plants, miRNAs negatively regulate mRNA targets by guiding a cleavage in the complementary site that leaves a 3 ¢ polyadenylated RNA possessing monophosphate at its 5 ¢ end. This chapter describes a detailed step-by-step protocol for cloning such sliced 3 ¢ products in order to identify small RNA targets. Using this protocol, we have identi fi ed more than 150 small RNA targets in rice; some are conserved and others are non-conserved targets for rice small RNAs.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SMALL RNA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MICRORNAS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MIRNA TARGETS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element POSTTRANSCRIPTIONAL GENE REGULATION
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Li, Y-F.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sunkar, R.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1i_JnxrEcSbuwBDKRNCGxIl6v_x0Tczzy/view?usp=drivesdk">https://drive.google.com/file/d/1i_JnxrEcSbuwBDKRNCGxIl6v_x0Tczzy/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
Holdings
Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-14695 25.06.2025 25.06.2025 Documentos solicitados