Cloning of cDNA encoding the sweet-tasting plant protein thaumatin and its expression in Eschericha coli (Record no. 48929)
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| fixed length control field | 01626nam a2200253Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625153947.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-14744 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Cloning of cDNA encoding the sweet-tasting plant protein thaumatin and its expression in Eschericha coli |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Gene, 18, p.1-12, 1982 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The structural gene of the sweet-tasting plant protein (prepro)thaumatin was cloned and expressed in Escherichia coli. Expression was effected under control of fuc and trp promoter/operator systems and through the use of bacterial ribosome-binding sites. The naturally occurring thaumatin II represents a processed form. The primary translation product, preprothaumatin, of the cloned mRNA-derived cDNA contains extensions at both the amino terminus and the carboxy terminus. The amino terminal extension of 22 amino acids is hydrophobic and very much resembles an excretion-related signal sequence. The six amino acids-long carboxy terminal extension is very acidic in character, in contrast to the overall highly basic thaumatin molecule. The possible role of such an acidic tail with respect to compartmentalization is discussed. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Edens, L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Heslinga, L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Klok, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ledeboer, A.M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Maat, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Toonen, M.Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Visser, C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Visser, C. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1SvWqJWe2473Ql2dOzElGibHc9zb1ynAl/view?usp=drivesdk">https://drive.google.com/file/d/1SvWqJWe2473Ql2dOzElGibHc9zb1ynAl/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-14744 | 25.06.2025 | 25.06.2025 | Documentos solicitados |