Study and Development of Multilayer Needle-type Enzyme-based Glucose Microsensors (Record no. 48940)

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control field 20250625153947.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-14755
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245 10 - TITLE STATEMENT
Title Study and Development of Multilayer Needle-type Enzyme-based Glucose Microsensors
490 0# - SERIES STATEMENT
Volume/sequential designation Biosensors, 4(1), p.27-40, 1989
520 3# - SUMMARY, ETC.
Summary, etc. Glucose oxidase (GOD)was covalently coupled to a cellulose acetate (CA)layer, using bovine serum albumin (BSA)and parabenzoquinone (PBQ)linkages, Prior to GOD coupling this CA layer was deposited on the platinum tip of a needle-type sensor and covered with an outer layer of polyurethane (PU). Such microsensors were found to be active, their GOD load reaching 1.6 to 3.0 pg mm-' and their glucose response reaching I to 3 PA M-' mme2, even when the upper limit of their linear range reached IO-30 mM. Due to the multilayer structure and composition of these micro- sensors, small anions such as ascorbate were partially discriminated from neutral molecules such as hydrogen peroxide. When implanted sub- cutaneously in anaesthetized rats, sensor responses correlated correctly with blood glucose concentration but presented sensitivity coejjicients signifi- cantly different to those determined in vitro: a 2 point calibration procedure was found necessary for in vivo experiments.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GLUCOSE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MICROSENSOR
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Topical term or geographic name entry element NEEDLE
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Topical term or geographic name entry element SUBCUTANEOUS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CELLULOSE ACE-TATE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element POLYURETHANE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GLUCOSE OXIDASE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PARABENZOQUIONE
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Personal name Stemberg, R.
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Personal name Barrau, M-B.
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Personal name Gangiotti, L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Thévenot, D.R.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1GfelgOp5Tnx2EfXG9LyUS0HeJjEzPlbh/view?usp=drivesdk">https://drive.google.com/file/d/1GfelgOp5Tnx2EfXG9LyUS0HeJjEzPlbh/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-14755 25.06.2025 25.06.2025 Documentos solicitados