A Species-Specific Polymerase Chain Reaction Assay for Rapid and Sensitive Detection of Colletotrichum capsici (Record no. 48957)

MARC details
000 -LEADER
fixed length control field 02132nam a2200289Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625153948.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-14772
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245 10 - TITLE STATEMENT
Title A Species-Specific Polymerase Chain Reaction Assay for Rapid and Sensitive Detection of Colletotrichum capsici
490 0# - SERIES STATEMENT
Volume/sequential designation Mol. Biotechnol, 49, p.48-55, 2011
520 3# - SUMMARY, ETC.
Summary, etc. Colletotrichum capsici is an important fungal species that causes anthracnose in many genera of plants causing severe economic losses worldwide. A primer set was designed based on the sequences of the ribosomal internal transcribed spacer (ITS1 and ITS2)regions for use in a conventional PCR assay. The primer set (CcapF/ CcapR)amplified a single product of 394 bp with DNA extracted from 20 Mexican isolates of C. capsici. The specificity of primers was confirmed by the absence of amplified product with DNA of four other Colletotrichum species and eleven different fungal genera. This primer set is capable of amplifying only C. capsici from different contaminated tissues or fungal structures, thereby facilitating rapid diagnoses as there is no need to isolate and cultivate the fungus in order to identify it. The sensitivity of detection with this PCR method was 10 pg of genomic DNA from the pathogen. This is the first report of a C. capsici-specific primer set. It allows rapid pathogen detection and provides growers with a powerful tool for a rational selection of fungicides to control anthracnose in different crops and in the post-harvest stage.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element COLLETORICHUM CAPSICI
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DIAGNOSTICSITS SEQUENCES
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ANTHRACNOSE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MOLECULAR DETECTION
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Torres-Calzada,C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Tapia-Tussell, R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Quijano-Ramayo, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Martin-Mex, R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Rojas-Herrera, R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Higuera-Ciapara, I.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Perez-Brito, D.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1DwcwITyR8EMiI27-aCKjh4XD6xNBw6qJ/view?usp=drivesdk">https://drive.google.com/file/d/1DwcwITyR8EMiI27-aCKjh4XD6xNBw6qJ/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-14772 25.06.2025 25.06.2025 Documentos solicitados