Examination of Mineralized Nodule Formation in Living Osteoblastic Cultures Using Fluorescent Dyes (Record no. 48974)
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| fixed length control field | 02028nam a2200205Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625153948.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-14789 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Examination of Mineralized Nodule Formation in Living Osteoblastic Cultures Using Fluorescent Dyes |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Biotechnology Progress, 22, p.1697-1701, 2006 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Detecting the formation of mineralized nodules in osteogenic cell culture provides a means of assessing mature osteoblast cell function and the status of culture. In the present study, to continuously monitor the formation of mineralized nodules during the entire culture period, different concentrations of two fluorescent dyes (xylenol orange and calcein blue)were evaluated for their ability to specifically label calcified areas and their toxicity to cells in osteogenic cultures. Results showed that 20íM xylenol orange and 30íM calcein blue gave rise to distinct fluorescent staining for mineralized nodules, which were correlated exactly with von Kossa and alizarin red S staining at the same locations in cultures. In the assessment of toxicity, both dyes at the aforementioned concentrations did not alter cell viability or change the total DNA content in cultures. To demonstrate the advantage of using these fluorochromes to monitor mineralized nodules formation, consecutive fluorescent images of each staining were recorded at the same location of individual culture over the entire duration. The result indicates that both xylenol orange and calcein blue can provide contrasting fluorescent staining to continuously monitor mineralized nodules formation in living osteogenic cell cultures without deleterious effects. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wang, Y.H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Liu, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Maye, P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rowe, S.W. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1EcQK2MJo8_XtokpzCfX6fujw_-UU4r9C/view?usp=drivesdk">https://drive.google.com/file/d/1EcQK2MJo8_XtokpzCfX6fujw_-UU4r9C/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-14789 | 25.06.2025 | 25.06.2025 | Documentos solicitados |