Transformation of Yeast by Lithium Acetate/Single-Stranded Carrier DNA/Polyethylene Glycol Method (Record no. 49038)

MARC details
000 -LEADER
fixed length control field 01552nam a2200181Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625153949.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-14853
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250602s9999 xx |||||s2 |||| ||und|d
245 10 - TITLE STATEMENT
Title Transformation of Yeast by Lithium Acetate/Single-Stranded Carrier DNA/Polyethylene Glycol Method
490 0# - SERIES STATEMENT
Volume/sequential designation Methods in Enzymology, 350, p.87-96, 2002
520 3# - SUMMARY, ETC.
Summary, etc. The introduction of exogenous DNA into yeast by transformation has become an essential technique in molecular biology. Transformation is used to investigate the genomics and proteomics of yeast itself and also when yeast is employed as a system to study the genes and gene products of other organisms. Intact yeast cells can be transformed by a number of procedures: the lithium acetate/single-stranded carrier DNA/polyethylene glycol (LiAc/SS-Carrier DNA/PEG)method, 1-3 elec- troporation, 4-6 agitation with glass beads, 7 and bombardment with DNA-coated microprojectiles. 8 Cells can also be transformed after conversion to spheroplasts by treatment with Zymolyase. 9 We have reviewed the application of these techniques to Saccharomyces cerevisiae and other yeastsJ ° We focus here on the transforma- tion of intact cells by the LiAc/SS-Carrier DNA/PEG method since it is the most widely applicable.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Daniel Gietz, R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Woods, R.A.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1cw4pbMAdASITt4wJSM9I7-HPdP5J4Hem/view?usp=drivesdk">https://drive.google.com/file/d/1cw4pbMAdASITt4wJSM9I7-HPdP5J4Hem/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
Holdings
Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-14853 25.06.2025 25.06.2025 Documentos solicitados