Purification of DNA from estuarine sediments (Record no. 49057)

MARC details
000 -LEADER
fixed length control field 01972nam a2200229Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625153949.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-14873
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
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245 10 - TITLE STATEMENT
Title Purification of DNA from estuarine sediments
490 0# - SERIES STATEMENT
Volume/sequential designation Journal of MicroBiological Methods, 20(3), p.161-174, 1994
520 3# - SUMMARY, ETC.
Summary, etc. DNA recovered from estuarine sediments after direct lysis of sediment microflora contains contaminants which interfere with restriction endonuclease digestion and other enzymic manipulations. Removal of these contaminants has proven excessively difficult and time consuming. New procedures were incorporated into established direct lysis methods in order to improve the quality of DNA recovered from sediments. Crude DNA preparations were recovered from estuarine sediment samples spanning a broad range of median grain sizes, silt and clay contents, and organic matter contents. The samples were incubated with ammonium acetate and ethidium bromide, extracted with phenol, and the aqueous DNA solutions isopropanol precipitated. These treatments greatly reduced contaminant quantity, as determined by spectrophotometric analysis, but did not yield restriction digestible DNA. This partially purified DNA was further purified using ion exchange chromatography, yielding DNA susceptible to restriction digestion. DNA purified using ion exchange chromatography was comparable in quality to that recovered from CsC1 gradient ultracentrifugation, and was obtained at substantial savings in time and expense compared to the latter method.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MICROBIAL DNA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DNA PURIFICATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ION EXCHANGE CHROMATOGRAPHY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SEDIMENT
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Lovell, C. R,
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Piceno Y.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1mXaOJTopv1zBFi65BpI7btQwvxMR_yNK/view?usp=drivesdk">https://drive.google.com/file/d/1mXaOJTopv1zBFi65BpI7btQwvxMR_yNK/view?usp=drivesdk</a>
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942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-14873 25.06.2025 25.06.2025 Documentos solicitados