Evaluation of regenerated strains from six Cucurbita interspecific hybrids obtained through anther and ovule in vitrocultures (Record no. 49080)
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| 000 -LEADER | |
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| fixed length control field | 02986nam a2200277Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625153950.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-14896 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Evaluation of regenerated strains from six Cucurbita interspecific hybrids obtained through anther and ovule in vitrocultures |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Australian Joournal of Crop Science, 6(1), p.23-30, 2012 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Homozygous plants can be obtained in a single generation using doubled haploid technology, which can tremendously facilitate breeding programs. The present study reports on in vitroregeneration and production of double haploid plants through anther and ovule cultures resulting from the hybridization of cultivated species of Cucurbita pepoL. with three other Cucurbita species i.e. C. moschataL., C. ficifoliaand C. martineziiL. Among six sucrose-2,4-Dichlorophenoxyacetic acid (2,4-D)combinations tested, the addition of 90 g l -1 sucrose and 1 mg l -1 2,4-D into the medium was found to be more suitable for callus regeneration from anthers. Anthers from the hybrid C. pepoL. (Queen F 1)× C. moschataL. regenerated the highest percentage (40 percent)of plantlets from callus and highest average number of plantlets per callus (7.5)when the culture medium was supplemented withthe aforementioned concentrations of sucrose and 2,4-D. Ovules collected from the hybrid C. pepoL. (MHTC77 F 1)× C. moschataL. produced the highest percentage of regenerated plantlets (25.33 percent), the highest percentage of responded ovules (18.67 percent)and the highest average number of plantlets per ovule (3.37). Cold pretreatment had negative effects on gynogenesis with the exception of the hybrid C. pepo L. (MHTC77 F 1)× C. moschataL. Chromosome counting in the root tips of androgenic plantlets revealed 50 percent of haploid and (n= x = 20)and 50 percent of dihaploid plantlets (2n= 2x= 40). The assessment of root tips from gynogenic plantlets resulted in 60 percent haploid (2n= x = 20)and 40 percent diploid (2n= 2x= 40)plants. The androgenic strains were evaluated for their vegetative and reproductive traits compared with open local cultivar C. pepoL. (Eskandarani cv.). While the majority of regenerated strains were intermediate between the cultivated species of C. pepoL. and other Cucurbita species, two regenerated strains proved superior to the local cultivar 'Eskandarani' in number of fruits (early and total yield/plot)as well as in total fruit yield per plot. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ANTHER CULTURE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CUCURBITACEAE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | GENOTYPE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | HAPLOID |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | OVULE CULTURE |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rakha, M.T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Metwally, E.I. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Moustafa,S.A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Etman, A.A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dewir, Y.H. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1WB3NuV11DLJmyrF1H11_t4QjoBtNTfP4/view?usp=drivesdk">https://drive.google.com/file/d/1WB3NuV11DLJmyrF1H11_t4QjoBtNTfP4/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-14896 | 25.06.2025 | 25.06.2025 | Documentos solicitados |