Methodological approaches for using synchrotron X-ray fluorescence (SXRF)imaging as a tool in ionomics: examples from Arabidopsis thaliana (Record no. 49111)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 02291nam a2200217Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625153951.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-14927 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Methodological approaches for using synchrotron X-ray fluorescence (SXRF)imaging as a tool in ionomics: examples from Arabidopsis thaliana |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Metallomics, 5, p.1133-1145, 2013 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Here we present approaches for using multi-elemental imaging (specifically synchrotron X-ray fluorescence microscopy, SXRF)in ionomics, with examples using the model plantArabidopsis thaliana.The complexity of each approach depends on the amount ofaprioriinformation available for the gene and/or phenotype being studied. Three approaches are outlined, which apply to experimental situations where a gene of interest has been identified but has an unknown phenotype (phenotyping), an unidentified gene is associated with a known phenotype (gene cloning)and finally, a screening approach, where both gene and phenotype are unknown. These approaches make use of open-access, online databases with which plant molecular genetics researchers working in the model plant Arabidopsis will be familiar, in particular the Ionomics Hub and online transcriptomic databases such as the Arabidopsis eFP browser. The approaches and examples we describe are based on the ssumption that altering the expression of ion transporters can result in changes in elemental distribution. We provide methodological details on using elemental imaging to aid or accelerate gene functional characterization by narrowing down the search for candidate genes to the tissues in which elemental distributions are altered. We use synchrotron X-ray microprobes as a technique of choice, which can now be used to image all parts of an Arabidopsis plant in a hydrated state. We present elemental images of leaves, stem, root, siliques and germinating hypocotyls. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Punshon, T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ricachenevsky, F.K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hindt, M.N. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Socha, A.L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zuber, H. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1QP5b9JJEJb8iT7CMpfgY4fjKuNBmabJ6/view?usp=drivesdk">https://drive.google.com/file/d/1QP5b9JJEJb8iT7CMpfgY4fjKuNBmabJ6/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-14927 | 25.06.2025 | 25.06.2025 | Documentos solicitados |