Phylogenomic analysis of Cation Diffusion Facilitator proteins uncovers Ni 2+/Co2+ transporters (Record no. 49112)
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| fixed length control field | 02033nam a2200205Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625153951.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-14928 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Phylogenomic analysis of Cation Diffusion Facilitator proteins uncovers Ni 2+/Co2+ transporters |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Metallomics, 5, p.1634-1643, 2013 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The ubiquitous Cation Diffusion Facilitator proteins (CDF)play a key role in maintaining the cellular homeostasis of essential metal ions. Previous neighbor-joining phylogenetic analysis classified CDF proteins into three substrate-defined groups: Zn 2+ ,Fe 2+ /Zn 2+ and Mn 2+ . These studies were unable to discern substrate-defined clades for Ni 2+ ,Co 2+ ,Cd 2+ and Cu 2+ transporters, despite their existence in this family. In this study we improved the accuracy of this previous functional classification using a phylogenomic approach based on a thorough maximum-likelihood phylogeny and the inclusion of recently characterized CDF transporters. The inference of CDF protein function predicted novel clades for Zn 2+ ,Fe 2+ ,Cd 2+ and Mn 2+ . The Ni 2+ /Co 2+ and Co 2+ substrate specificities of two clades containing uncharacterized proteins were defined through the functional characterization ofnepAandcepAmetal inducible genes which independently conferred Ni 2+ and Co 2+ resistances to Rhizobium etliCFN42 and increased, respectively, Ni 2+ /Co 2+ and Co 2+ resistances Escherichia coli. Neither NepA nor CepA confer Zn 2+ ,Fe 2+ and Mn 2+ resistances. The ability of NepA to confer Ni 2+ /Co 2+ resistance is dependent on clade-specific residues Asn 88 and Arg 197 whose mutations roduce a non-functional protein. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Cubillas, C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Vinuesa, P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Tabche, M.L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | García-De Los Santos, A. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/19wyqTzXdvjQ4EP4wHpRwCkysv3_cejy2/view?usp=drivesdk">https://drive.google.com/file/d/19wyqTzXdvjQ4EP4wHpRwCkysv3_cejy2/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-14928 | 25.06.2025 | 25.06.2025 | Documentos solicitados |