PCR Detection ofAlternariaspp. in Processed Foods, Based on the Internal Transcribed Spacer Genetic Marker (Record no. 49148)

MARC details
000 -LEADER
fixed length control field 02009nam a2200229Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625153951.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-14965
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250602s9999 xx |||||s2 |||| ||und|d
245 10 - TITLE STATEMENT
Title PCR Detection ofAlternariaspp. in Processed Foods, Based on the Internal Transcribed Spacer Genetic Marker
490 0# - SERIES STATEMENT
Volume/sequential designation Journal of Food Protection, 74(2), p.240-247, 2011
520 3# - SUMMARY, ETC.
Summary, etc. The genusAlternariais considered one of the most important fungal contaminants of vegetables, fruits, and cereals, producing several mycotoxins that can withstand food processing methods. Conventional methods for Alternariaidentification and enumeration are laborious and time-consuming, and they might not detect toxigenic molds inactivated by food processing. In this study, a PCR method has been developed for the rapid identification of Alternariaspp. DNA in foodstuffs, based on oligonucleotide primers targeting the internal transcribed spacer (ITS)1 and ITS2 regions of the rRNA gene. The specificity of the Alternaria-specific primer pair designed (Dir1ITSAlt-Inv1ITSAlt)was verified by PCR analysis of DNA from various Alternariaspp., and also from several fungal, bacterial, yeast, animal, and plant species. The detection limit of the method was 10 2 CFU/ml in viable culture, heated culture, or experimentally inoculated tomato pulp. The applicability of the method for detection ofAlternariaspp. DNA in foodstuffs was assessed by testing several commercial samples. AlternariaDNA was detected in 100 (percent)of spoiled tomato samples, 8(percent)of tomato products, and 36.4(percent)of cereal-based infant food samples analyzed.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Pavon, M.A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Gonzalez, I.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Rojas, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Pegels, N.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Martin, R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Garcia,T.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1IuDlU0iv6pGwHY_scoCT1uCRv-SEEr3J/view?usp=drivesdk">https://drive.google.com/file/d/1IuDlU0iv6pGwHY_scoCT1uCRv-SEEr3J/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
Holdings
Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-14965 25.06.2025 25.06.2025 Documentos solicitados