An agrobacterium-mediatesd transie4nt gene expression system for intact leaves (Record no. 49192)

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control field 20250625153952.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-15010
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Title An agrobacterium-mediatesd transie4nt gene expression system for intact leaves
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Volume/sequential designation Plant Science , 122(1), p.101-108, 1997
520 3# - SUMMARY, ETC.
Summary, etc. An efficient and reproducible AgrohLlc~teriut,l-mediated transient gene expression system for intact leaf tissue was developed. A high level of transient expression was observed when bacteria. which were pretreated in C?Y gene-induc- ing conditions. were infiltrated into complete leaf tissue. Histochemical /I-glucuronidase assays showed large transgene-expressing sectors comprising of up to 90 percent of the leaf area. As a consequence of infiltration, the induced bacteria entered into the intercellular spaces, thus enabling T-DNA transfer in all cell layers of the leaf. The protocol was optimized for Phasrolus wlgaris leaves. but similar results were obtained with other plant species. such as P/mroh~.s uc,utifdim. poplar, and tobacco. A /I-glucuronidase chimeric gene interrupted by an intron was used as a marker for histological detection of the sectors. Copyright :CJ 1997 Elsevier Science Ireland Ltd.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kapila, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Riet De Rycke,
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Marc Van Montagu
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Angenon, G.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/1I2DtrAD2AaMuCTwLqQMQ0-gdfGCupocE/view?usp=drivesdk">https://drive.google.com/file/d/1I2DtrAD2AaMuCTwLqQMQ0-gdfGCupocE/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-15010 25.06.2025 25.06.2025 Documentos solicitados