Cloning and characterization of GPD2, a second gene encoding sn-glycerol 3-phosphate dehydrogenase (NAD +)in Saccharomyces cerevisiae, and its comparison with GPD1 (Record no. 49225)
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| fixed length control field | 02129nam a2200217Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625153953.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-15043 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Cloning and characterization of GPD2, a second gene encoding sn-glycerol 3-phosphate dehydrogenase (NAD +)in Saccharomyces cerevisiae, and its comparison with GPD1 |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Molecular MicroBiology, 17(1), p.95-107, 1995 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | We have cloned and characterized a homologue of the previously isolated GPD1 gene, encoding sn-glycerol 3-phosphate dehydrogenase (NAD +)in Saccharo- myces cerevisiae. This second gene, called GPD2, encodes a protein of 384 amino acids that shares 69 percent sequence identity with GPD1. Like GPD1 it has an amino-terminal extension of unknown function. GPD2 is located on chromosome VII and cross- hybridizes with GPD1 at chromosome IV as well as with an unknown homologue at chromosome XV. Dis- ruption of the GPD2 gene did not reveal any observ- able phenotypic effects, whereas overexpression resulted in a slight, but significant, increase of GPD enzyme activity in wild-type cells. Analysis of gene transcription by a CAT-reporter gene fused to the GPD promoters revealed decreased transcriptional activity of the GPD2 promoter in cells grown on non- fermentable as opposed to fermentable carbon sources, and no induction in cells exposed to high osmolarity or heat shock. Similar analysis of GPD1 demonstrated an 8-17-fold higher basal level of transcription compared to GPD2. Furthermore, such analysis revealed that the GPD1 promoter was induced by increased osmolarity essentially indepen- dent of the type of stress solute used, the level of GPD1 transcription being increased about sevenfold in cells growing at 1.4 M NaCI. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Eriksson, P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Andre, L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ansell, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Blomberg, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Adler, L. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1TPDlPotyB10CqblFy7ZSi3J7tJc3Cuzz/view?usp=drivesdk">https://drive.google.com/file/d/1TPDlPotyB10CqblFy7ZSi3J7tJc3Cuzz/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-15043 | 25.06.2025 | 25.06.2025 | Documentos solicitados |